Thermoneutrality Exacerbates Nonalcoholic Steatohepatitis in Mice

Student(s): James Rescinito, Md Shahjalal Hossian Khan, Shu Wang

Mentor(s): Lei Hao

Abstract:

Objectives:

Nonalcoholic Steatohepatitis (NASH), the aggressive subtype of nonalcoholic fatty liver disease, affects about 1.5-6.45% of population in the world. Understanding the pathophysiology of NASH is critical to develop a therapeutic strategy for NASH. In this study, we investigated whether brown fat activation can modulate the progression of NASH.

Methods:

Male C57BL/6J mice were randomly assigned to 2 groups (16 mice/group), and fed with either a control chow diet (CHD) (Picolab rodent diet 20, LabDiet) with a regular drinking water or an “fast food” diet (FFD) (Research Diets, D12079B) plus fructose 23.1 g/L and glucose 18.9 g/L added to the drinking water for 20 weeks. Half of mice in each group were housed at the standard temperature (ST) (22OC), and the other half of mice were housed at the thermoneutral temperature (TT) (27OC).

Results:

We found that brown fat activation via cold housing improved the progression of NASH in mice.

Presentation:

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